LOCUS       sequence-55597-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Cheng KC, Inglese J
  TITLE     A coincidence reporter-gene system for high-throughput screening.
  JOURNAL   Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
  PUBMED    23018994
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2012-10"; volume: "9(10):937. doi"; pages: " 
            10.1038/nmeth.2170"
COMMENT     Sequence Label: pCI-6.24
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(75..105)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    263..354
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(295..314)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    368..416
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(684..705)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct taagctggaa gtcgagcttc cttatatacc ctctagtgtc tgaattcctc
      121 tgacgtcagg ctctctgacg tcaggctctc tgacgtcagg ctctctgacg tcaggctctt
      181 agatcgcaga tctcgagcaa gcttacttag atccagatct cgagcccggg ctagcacgcg
      241 taagagctcg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      301 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      361 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      421 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
      481 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      541 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      601 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      661 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      721 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      781 caccctaatc aagttttt
//
