LOCUS       sequence-55598-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YFP-PIF for red-light-induced binding to Phy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL175
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(338..356)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(339..356)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(376..393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    382..606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(739..758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgatgttct taccaaccga ttattgttgc aggttaagcg atcaagagta tatggagctt
       61 gtgtttgaga atggccagat tcttgcaaag ggccaaagat ccaacgtttc tctgcataat
      121 caacgtacca aatcgatcat ggatttgtat gaggcagagt ataacgagga tttcatgaag
      181 agtatcatcc atggtggtgg tggtgccatc acaaatctcg gggacacgca ggttgttcca
      241 caaagtcatg ttgctgctgc ccatgaaaca aacatgttgg aaagcaataa acatgttgac
      301 taagcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
      361 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      421 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      481 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
      541 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
      601 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
      661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      781 gctttccccg tcaagctcta aatcgggggc tcccctttag ggttccgatt tagtgcttta
      841 cggcacctcg accccaaaaa acttgatta
//
