LOCUS       sequence-55601-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Cheng KC, Inglese J
  TITLE     A coincidence reporter-gene system for high-throughput screening.
  JOURNAL   Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
  PUBMED    23018994
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2012-10"; volume: "9(10):937. doi"; pages: " 
            10.1038/nmeth.2170"
COMMENT     Sequence Label: pCI-6.22
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    121..212
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(153..172)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    226..274
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(542..563)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     754..773
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg aatgccaagc
       61 ttacttagat ccagatctcg agcccgggct agcacgcgta agagctcggt acctatcgat
      121 agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt
      181 gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa
      241 cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta cctcccgtac
      301 cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag aggcccgcac
      361 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa
      421 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      481 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      541 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      601 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
      661 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      721 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      781 taggc
//
