LOCUS       sequence-55612-e       11342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo single-strand annealing substrate composed of two truncated 
            GFP genes, separated by a drug selection marker, and flanked by 
            mouse hprt targeting sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M
  TITLE     Genetic steps of mammalian homologous repair with distinct mutagenic
            consequences.
  JOURNAL   Mol Cell Biol. 2004 Nov;24(21):9305-16.
  PUBMED    15485900
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-11"; volume: "24(21)"; pages: "9305-16"
COMMENT     Sequence Label: hprtSAGFP
FEATURES             Location/Qualifiers
     source          1..11342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2223..2239
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        3565..3944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3947..4222
                     /label=chicken beta-actin promoter
     intron          4223..5231
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5155..5177
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5239..5258
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5286..5295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5292..5294,5295..5297,5298..5810)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(5337..5358)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        6048..6545
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6083..6106)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6478..6497
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6564..7163
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6564..6583)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7060..7080
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7621..7640)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7686..7741
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7740..7759)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     8672..8693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8898..8917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10698..10717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10699..10717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10724..10741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10724..10740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10733..10755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10882..11337
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10969..10988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11179..11200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtggatcc gcggccgcta attcgattct tctgtaaaat tatcttatta
     2281 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg
     2341 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg
     2401 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta
     2461 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc
     2521 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt
     2581 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc
     2641 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt
     2701 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa
     2761 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag
     2821 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt
     2881 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat
     2941 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta
     3001 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg
     3061 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg
     3121 acagccagag ctatacagag aaatcctgtc tcaaaaaacc aaaaataaag tgtatatact
     3181 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt
     3241 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact
     3301 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg
     3361 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt
     3421 attatctaaa cgtaattaaa tgttgatatt attgaagcat ttatcagggt tattgtctca
     3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3541 ttccccgaaa agtgccacct ggtcgacatt gattattgac tagttattaa tagtaatcaa
     3601 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3661 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3721 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3781 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     3841 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     3901 ctacttggca gtacatctac gtattagtca tcgctattac catgggtcga ggtgagcccc
     3961 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     4021 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg
     4081 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     4141 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4201 aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg
     4261 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     4321 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc
     4381 ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga
     4441 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct
     4501 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc
     4561 gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag
     4621 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc
     4681 tgtaaccccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     4741 gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     4801 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     4861 ggccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4921 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg
     4981 gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag
     5041 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag
     5101 cctcggggct gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     5161 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     5221 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     5281 aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5341 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5401 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5461 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5521 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5581 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5641 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5701 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5761 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5821 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5881 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagtgag
     5941 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     6001 tggctcacaa ataccactga gatctgatat catcgatgaa ttctaccggg taggggaggc
     6061 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6121 cacaagtggc ctctggcctc acacattcca catccaccgg taggcgccaa ccggctccgt
     6181 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     6241 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     6301 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     6361 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     6421 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     6481 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     6541 tttcgaccga tccagccgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6601 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6661 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6721 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6781 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6841 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6901 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6961 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7021 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7081 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7141 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     7201 gcgcacgacc ccatggctcc gaccgaagcc acccggggcg gccccgccga ccccgcaccc
     7261 gcccccgagg cccaccgcgg gggacacacc gaacacgccg accctgctga acacgcggcg
     7321 cagttcggtg cccaggagcg gatcgaaatt gatgatctat taaacaataa agatgtccac
     7381 taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat
     7441 tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg
     7501 ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata
     7561 atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga
     7621 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     7681 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     7741 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     7801 gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc
     7861 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     7921 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     7981 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     8041 ctgtccctct tctcttatga agatccctcg acctgcagcc caagctttaa ggccacaagt
     8101 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     8161 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     8221 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     8281 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     8341 agacccgcgc cgaggtgaag ttcgataggg ataacagggt aatccgcatc gagctgaagg
     8401 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     8461 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     8521 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     8581 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     8641 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     8701 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     8761 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     8821 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     8881 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8941 gaagcttgtt tttatttgta tctttaaatt ttatgataga tgttatagtg tactctcctc
     9001 tcccctcccc tcccctctcc tcctcagcag gatcttgtta tgtagcccta agggatctaa
     9061 tacttactct gtagtccagg ctgtccttca gctcatgaga cacaccatca tgcttggcat
     9121 gtttcttgtt ctgaaaggta tctggtttta attttggttg agctggtgtg ttggtgtata
     9181 tctgtaatct cagcacaggc aagtagagac aaggattatg attacagtcc agcctgggct
     9241 atttagaggg attctcttta atctttcctc atgccccaaa atcttacctt tggtatatga
     9301 aaaatagtct ccacttctgc aaaatattgc tttatgaagt aagaattccc ttcatagaga
     9361 caaggaatgt gtcctgtaaa agtttaatgt gtaagaagta tttgttataa aagataaata
     9421 ttcagaatct tctttttaat tcctgatttt atttctatag gactgaaaga cttgctcgag
     9481 atgtcatgaa ggagatggga ggccatcaca ttgtggccct ctgtgtgctc aaggggggct
     9541 ataagttctt tgctgacctg ctggattaca ttaaagcact gaatagaaat agtgatagat
     9601 ccattcctat gactgtagat tttatcagac tgaagagcta ctgtgtaagt ataattaact
     9661 tataattaaa aaaatagggc cattctagtt ttatctatat tttttttaaa cttgtgcaaa
     9721 ctatgctaca taatcaactt acttgattat gtaatatata tagtaatagt tggaattact
     9781 attattacat atttattttt ctgtgctggg gtagaaccca gggaactaag tactgtaata
     9841 accgagcttc attcatccta gtcattttta attttttttt ttttttttgt tttttcgaga
     9901 caaggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct
     9961 cgaactcaga aatctgcctg cctctgcctc ccagtgctga gattaatgac gtgcaccacc
    10021 actgcctggc atttttttaa agttttaatt tgagaccctt tctcactaac ttgctcacac
    10081 tatcctcgaa ctcattacgt gggctggtct agaaatttca gtcaacttgc ctcagcttct
    10141 caagtagctg ggattacagg caggtgctac cacttctggc catgatcatt gttgttgttg
    10201 ctattattat tattattatt tcactgatac ttcatatcac aacacagaat agtaactgta
    10261 gttcatgacc aacaaaaaag ttgtacagtt tctctgttgt cttattttga tctggatttt
    10321 gatttgtttc atggcactgg gcaggctaat ctcaaactgc atggctcaaa tagtccttct
    10381 gtcttaatct cccatgttct ggaagtatag gtatcactac acctagctgt attttcatat
    10441 tttaaaaaaa acgcctaaga attgctattg aatgaggcgg agagatggct cagcagtcac
    10501 aagtacttgt agaggactgg ggtttggtta ctagcacctc aaagatggct tacaattatc
    10561 tgtagctcca attccagagg atctgtgtgg taaagctgca tgtatatggg catgcaggta
    10621 aaactgctaa acatataaaa atagctcagt cttaagagaa ttggtcgacc tcgagggggg
    10681 gcccggtacc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
    10741 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    10801 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    10861 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    10921 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    10981 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    11041 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    11101 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    11161 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    11221 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    11281 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
    11341 tg
//
