LOCUS       sequence-55619-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DOT1L
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcctctagaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcatggg ggagaagctg gagctgagac
      121 tgaagtcgcc cgtgggggct gagcccgccg tctacccgtg gccgctgccg gtctacgata
      181 aacatcacga tgctgctcat gaaatcatcg agaccatccg atgggtctgt gaagaaatcc
      241 cggatctcaa gctcgctatg gagaattacg ttttaattga ctatgacacc aaaagcttcg
      301 agagcatgca gaggctctgc gacaagtaca accgtgccat cgacagcatc caccagctgt
      361 ggaagggcac cacgcagccc atgaagctga acacgcggcc gtccactgga ctcctgcgcc
      421 atatcctgca gcaggtctac aaccactcgg tgaccgaccc cgagaagctc aacaactacg
      481 agcccttctc ccccgaggtg tacggggaga cctccttcga cctggtggcc cagatgattg
      541 atgagatcaa gatgaccgac gacgacctgt ttgtggactt ggggagcggt gtgggccagg
      601 tcgtgctcca ggttgctgct gccaccaact gcaaacatca ctatggcgtc gagaaagcag
      661 acatcccggc caagtatgcg gagaccatgg accgcgagtt caggaagtgg atgaaatggt
      721 atggaaaaaa gcatgcagaa tacacattgg agagaggcga tttcctctca gaagagtgga
      781 gggagcgaat cgccaacacg agtgttatat ttgtgaataa ttttgccttt ggtcctgagg
      841 tggatcacca gctgaaggag cggtttgcaa acatgaagga aggtggcaga atcgtgtcct
      901 cgaaaccctt tgcacctctg aacttcagaa taaacag
//
