LOCUS       sequence-55621-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PIAS2
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctctagaaat aattttgttt aactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggccagt taaaaaatct gcccttttat
      121 gatgtccttg atgttctcat caagcccacg agtttagttc aaagcagtat tcagcgattt
      181 caagagaagt tttttatttt tgctttgaca cctcaacaag ttagagagat atgcatatcc
      241 agggattttt tgccaggtgg taggagagat tatacagtcc aagttcagtt gagactttgc
      301 ctggcagaga caagttgccc tcaagaagat aactatccaa atagtctatg tataaaagta
      361 aatgggaagc tatttccttt gcctggctat gcaccaccgc ctaaaaatgg gattgaacag
      421 aagcgccctg gacgcccctt gaatattaca tctttagtta ggttatcttc agctgtgcca
      481 aaccaaattt ccatttcttg ggcatcagaa attgggaaga attactctat gtctgtatat
      541 cttgtacggc agcttacatc agccatgtta ttacagagat taaaaatgaa aggtattaga
      601 aaccctgatc attccagagc actaattaaa gaaaaactta ctgcagatcc tgatagtgaa
      661 attgctacaa ctagccttcg ggtatccttg atgtgccctt taggaaaaat gaggctgaca
      721 atcccatgcc gtgcagtgac ttgtacacat ctgcagtgtt ttgatgctgc cctctatcta
      781 caaatgaatg agaaaaagcc cacctggatt tgtcctgtgt gtgacaaaaa agctgcctat
      841 gaaagtctaa tattagatgg gctttttatg gaaattctca atgactgttc tgatgtagat
      901 gagatcaaat tccaagaaga tggttcttgg tgtccaatga gaccgaagaa agaagctatg
      961 aaagtatcca gccaaccgtg tacaaaaata gaaagttcaa gcgtcctcag taagccttgt
     1021 tca
//
