LOCUS       sequence-55623-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PP-MetAP
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attccctcta gaatattttg tttactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcgc catgaaaacg tttgacttca
      121 ccggaccatt gcggccgggt aagataacgc cacgccgcgc tgttccgtca catattctgc
      181 gaccagacta tgctgaccgt gcagggggcg tgtctgcctc agaggaaaaa gaccgaggaa
      241 gtaaggtgaa ggtatataat attcagttcc ttcacgatga cagcaaaaag actgcggaaa
      301 ttcagcgaat caagactgtt tgtcagctct cgcgggaggt gcttgacatt gcgacggcag
      361 cggccaaacc tggcatcacc accgatgagt tggaccgcat agtgcatgaa gccactgtcg
      421 agcgtaacat gtacccgtct ccgctaaatt actacggatt cccaaaatct gtgtgtacat
      481 ctgtgaatga agttatttgc catggtatcc ctgactctcg tgaactcgag gagggagaca
      541 tcttaaatat tgatgtttca agctacctca atggattcca cggtgacctg aacgagacgg
      601 tattcattgg tcgaccggat gatgatagcg tccggcttgt gcacgctgct tacgagtgtc
      661 tctgcgctgg cattggtgta gtgaaacctg aagcccttta caagcaggta ggtgatgcca
      721 ttgaggcctg tgcttcacaa tatcaatgct cagtggttcg cacctacacg ggtcatggtg
      781 ttggccacct cttccataca tcacctacgg tgtgtcacta cgccaacaac aagagtcttg
      841 gcatgatgcg gccagggcat gtcttcacga ttgagccgat gatcaactta ggaacatggc
      901 aggatgtaac atggcctgat aaatggacaa gtaccactaa agacgggcgc cgaagcgcac
      961 aatttgaaca cacga
//
