LOCUS       sequence-55625-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PP-ASL
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcatgg agaagggttc accatccgac ttaaacggtg
      121 tggattattc cgtggataat cccctcttcg ctctctcgcc actggacggt agatacaaac
      181 gtcaaacgaa ggcgctacgg gccttctttt ctgagtatgg gttctttaga taccgggtgt
      241 tggtggaggt tgaatacttc acggcgctct gtaaggatgt gcccaccatt gttccattac
      301 gaagtgtgac tgatgagcag ctgcagaagc tgcgcaaaat cacactggat tgcttttctg
      361 ttagtagtgc agaggaaatc aagaggttgg agcgcgtaac caaccatgat atcaaagccg
      421 tggagtactt tatcaaggaa cgtatggata cttgtggact tagtcacgtc actgagtttg
      481 tgcacttcgg tttgacatca caggacataa acaatacggc gattccaatg atgattcgtg
      541 atgctattgt aaccttgtac ctccctgcac tcgatgggat cattggctca cttacctcaa
      601 agcttgttga ttgggatgta ccgatgctcg ctcggacgca tggtcaaccc gcttcgccaa
      661 caaacctcgc gaaggagttt gtggtttgga tagagcggct acgcgagcaa cggcgacaac
      721 tgtgtgaagt acccacgact ggaaaatttg gaggtgccac cggaaacttc aacgctcacc
      781 tagtggcata tccatctgtg aactggcgcg cctttgctga tatgtttctg gccaaatatc
      841 tcggactgaa acgacagcaa gccaccacgc aaattgagaa ttatgaccat ttggccgcgc
      901 tgtgcgatgc atgcgcccgt ttgcacgtta ttctcattga tatgtgccgt gatgtgtggc
      961 agtacatctc tatggggttc ttcaagca
//
