LOCUS       sequence-55626-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Toronto SGC Q1 Q2 2012
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PP-LeuAP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcctctag aatattttgt ttaactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcat gcctacttta cccaaggccg
      121 aagcaaaaga gctttctgcg ttcgtccagt cgtgcgtcga atacaaaacc aatgtttgct
      181 tcaccgatgt cgccgcctac gaaagcaacc agaagggtgt actgtcctca ggactcgctg
      241 ttcttgttgg aactcataag caactccgtg accccgccgt ccagcgttta ccattttaca
      301 atcccgctgt tgctgaagct attgagcggg tgaaggaagg tgggacgtac ggcgttttgg
      361 tggagggact tgcgaatgcg gcaggctcaa agttcgtgcg cgttgtcgtt ggtgaggtgc
      421 ccacgaaggc atctcgcaac aactgccctg cccgaccgga tgttgtgact gcacttgtaa
      481 ctgctgcatt ggacgaagtt aaagagccta atacaacggt ggatgtcttt gtgctttcca
      541 atgctgtcct tcccatcgct gctgctgtag cacgctgtgg aaagcacaac ttctccgcaa
      601 aggatggtgc ggcagcggcg gcttacaaca gtggtaaggt cagccggttg caggtggtgt
      661 tcccggagcc gcccgcgatc ccgccaaagg acttggaggc ggttgccact tcgacacaac
      721 tctgccagcg tcttgttgac gcgcctccca acctgctaac cactgcaacc ttcacagaaa
      781 ttgcgcaagg ctacgcaaag gcgcttggct tcgatgtgga cgtcatttgt ggtgacgatt
      841 tgtgtgagcg cggctacgga ggcatataca gtgtgggaaa ggctgc
//
