LOCUS       sequence-55627-e        3825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        113..456
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             457..1155
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      1159..1356
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1460..1479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1461..1479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1566..1583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1737..2325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1817..1836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2496..3287,3288..3356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3119..3138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3357..3461)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3529..3547
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3585..3607)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3707..3726
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3807..3825
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3807..3824
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag
       61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
      181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
      241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
      301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
      361 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
      421 atacagttct cacatcacat ccgaacataa acaaccatgg cagaaccagc ccaaaaaaag
      481 caaaaacaaa ctgttcagga gcgcaaggcg tttatctccc gtatcactaa tgaaactaaa
      541 attcaaatcg ctatttcgct gaatggtggt tatattcaaa taaaagattc gattcttcct
      601 gcaaagaagg atgacgatgt agcttcccaa gctactcagt cacaggtcat cgatattcac
      661 acaggtgttg gctttttgga tcatatgatc catgcgttgg caaaacactc tggttggtct
      721 cttattgttg aatgtatcgg tgacctgcac attgacgatc accatactac cgaagattgc
      781 ggtatcgcat tagggcaagc gttcaaagaa gcaatgggtg ctgtccgtgg tgtaaaaaga
      841 ttcggtactg ggttcgcacc attggatgag gcgctatcac gtgccgtagt cgatttatct
      901 aatagaccat ttgctgtaat cgaccttgga ttgaagagag agatgattgg tgatttatcc
      961 actgaaatga ttccacactt tttggaaagt ttcgcggagg cggccagaat tactttgcat
     1021 gttgattgtc tgagaggttt caacgatcac cacagaagtg agagtgcgtt caaggctttg
     1081 gctgttgcca taagagaagc tatttctagc aatggcacca atgacgttcc ctcaaccaaa
     1141 ggtgttttga tgtgaagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt
     1201 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
     1261 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
     1321 tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc
     1381 catccagttt aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg
     1441 gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac
     1501 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1561 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1621 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1921 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2161 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2401 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2461 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2521 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2581 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2641 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2701 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2761 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2821 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2881 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2941 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3001 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3061 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3121 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3181 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3241 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3301 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3361 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3421 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3481 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3541 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3601 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3661 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3721 attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac
     3781 ataaccttat gtatcataca catacgattt aggtgacact ataga
//
