LOCUS       sequence-55630-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(131..154)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(185..436)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(227..248)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     576..595
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact
       61 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtctagata
      121 tctcgactca cttgtcatcg tcgtccttgt aatctccgcc agatcctggt gttccgccac
      181 gcgtcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      241 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt
      301 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      361 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      421 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      481 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      541 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      601 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      661 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga
      721 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      781 cgta
//
