LOCUS       sequence-55631-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2dW-Flag
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(133..156)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg gccctctaga
       61 ctcgagcggc cgccactgtg ctggatatca accactttgt acaagaaagc tgggtctaga
      121 tatctcgact cacttgtcat cgtcgtcctt gtaatctccg ccagatcctg gtgttccgcc
      181 acgcgtgtcc atagcgtctg ccccagtgat ggggatggga gccagttctc caatgggttc
      241 tagggacagg tgtggccagc ttcctttccc aggaaacatc accctctcct ggacgggaac
      301 atatatcttc ttcaatatgc gctccttctt ctggcgggcc cagagcatcc tctccagtcc
      361 ttgactcacc agatctgctc tgaatctgct gtccaaaacc ttccaccaaa cttggtgttt
      421 cctcctcttg ctctgctctg cctgagcctg ctcaccactg ctctcattct cagctgtgtg
      481 gtcggagtgc ttctcactga ctgcgtccag cagggccagt agctgaggct gtgaagccgc
      541 tggaagcacc acactcagga gtcgcagtag tgtgctgcct ggtaccattg tcatccttga
      601 gaggtatgac accagcctca gtccctggct gcaaaggctg gccatggtgt cagcttcaat
      661 gctttccaga gaatcttcca gcaccgcacc gttctgccac tcctctctca ccctctgctg
      721 atgctgctcc agcagcttct ggcccttgtt cagggcttgc cgcaggatgg cagagacctg
      781 cctttcagga t
//
