LOCUS       sequence-55636-e        4590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-13Myc-His3MX6
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..561
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      616..803
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(671..695)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        878..1221
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1222..1920
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      1924..2121
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2225..2244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2226..2244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2331..2348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2502..3090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2582..2601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3261..4052,4053..4121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3884..3903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4122..4226)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4294..4312
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4350..4372)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4472..4491
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4572..4590
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4572..4589
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaac
       61 ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa
      121 gaagacttga acggactcga cggtgaacaa aagttgattt ctgaagaaga tttgaacggt
      181 gaacaaaagc taatctccga ggaagacttg aacggtgaac aaaaattaat ctcagaagaa
      241 gacttgaacg gactcgacgg tgaacaaaag ttgatttctg aagaagattt gaacggtgaa
      301 caaaagctaa tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac
      361 ttgaacggac tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa
      421 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
      481 aacggactcg acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag
      541 ctaatctccg aggaagactt gaacggtgaa caaaaattaa tcaatcacta gtgaattcgc
      601 gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
      661 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
      721 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct
      781 cttattgacc acacctctac cggcagatcc gctagggata acagggtaat atagatctgt
      841 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
      901 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
      961 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1021 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1081 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1141 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1201 cacatccgaa cataaacaac catggcagaa ccagcccaaa aaaagcaaaa acaaactgtt
     1261 caggagcgca aggcgtttat ctcccgtatc actaatgaaa ctaaaattca aatcgctatt
     1321 tcgctgaatg gtggttatat tcaaataaaa gattcgattc ttcctgcaaa gaaggatgac
     1381 gatgtagctt cccaagctac tcagtcacag gtcatcgata ttcacacagg tgttggcttt
     1441 ttggatcata tgatccatgc gttggcaaaa cactctggtt ggtctcttat tgttgaatgt
     1501 atcggtgacc tgcacattga cgatcaccat actaccgaag attgcggtat cgcattaggg
     1561 caagcgttca aagaagcaat gggtgctgtc cgtggtgtaa aaagattcgg tactgggttc
     1621 gcaccattgg atgaggcgct atcacgtgcc gtagtcgatt tatctaatag accatttgct
     1681 gtaatcgacc ttggattgaa gagagagatg attggtgatt tatccactga aatgattcca
     1741 cactttttgg aaagtttcgc ggaggcggcc agaattactt tgcatgttga ttgtctgaga
     1801 ggtttcaacg atcaccacag aagtgagagt gcgttcaagg ctttggctgt tgccataaga
     1861 gaagctattt ctagcaatgg caccaatgac gttccctcaa ccaaaggtgt tttgatgtga
     1921 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     1981 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     2041 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     2101 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtttaaacg
     2161 agctcgaatt catcgatgat atcagatcca ctagtggcct atgcggccgc ggatctgccg
     2221 gtctccctat agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc
     2281 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2941 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4261 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4321 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4381 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4441 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     4501 caccatatgg acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
     4561 atacacatac gatttaggtg acactataga
//
