LOCUS       sequence-55651-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2ala4-Flag
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(124..147)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag actcgagcgg
       61 ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggtctag atatctcgac
      121 tcacttgtca tcgtcgtcct tgtaatctcc gccagatcct ggtgttccgc cacgcgtgtc
      181 caagcccaag gccctgttgg ccttcttggc attcaggaaa ttctttttcc tcctgggagg
      241 aacacgcaaa gagatctcgg gctcccttgg gcttctgaat gcagcggcag cttctcccgt
      301 gtttaatatg tccatagcgt ctgccccagt gatggggatg ggagccagtt ctccaatggg
      361 ttctagggac aggtgtggcc agcttccttt cccaggaaac atcaccctct cctggacggg
      421 aacatatatc ttcttcaata tgcgctcctt cttctggcgg gcccagagca tcctctccag
      481 tccttgactc accagatctg ctctgaatct gctgtccaaa accttccacc aaacttggtg
      541 tttcctcctc ttgctctgct ctgcctgagc ctgctcacca ctgctctcat tctcagctgt
      601 gtggtcggag tgcttctcac tgactgcgtc cagcagggcc agtagctgag gctgtgaagc
      661 cgctggaagc accacactca ggagtcgcag tagtgtgctg cctggtacca ttgtcatcct
      721 tgagaggtat gacaccagcc tcagtccctg gctgcaaagg ctggccatgg tgtcagcttc
      781 aatgctttcc agagaatctt ccagcaccgc accgttctgc cactcctctc tcaccctctg
      841 ctgatgctgc tc
//
