LOCUS       sequence-55652-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2ala5-Flag
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(122..145)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaggagaggg ttagggatag gcttaccttc gaaccgcggg ccctctagac tcgagcggcc
       61 gccactgtgc tggatatcaa ccactttgta caagaaagct gggtctagat atctcgactc
      121 acttgtcatc gtcgtccttg taatctccgc cagatcctgg tgttccgcca cgcgtgtcca
      181 agcccaaggc cctgttggcc ttcttggcat tcaggaaatt ctttttcctc ctgggaggaa
      241 cacgcaaaga gatctcgggc tcccttgggc tggcagctgc agcgatcttt tctcccgtgt
      301 ttaatatgtc catagcgtct gccccagtga tggggatggg agccagttct ccaatgggtt
      361 ctagggacag gtgtggccag cttcctttcc caggaaacat caccctctcc tggacgggaa
      421 catatatctt cttcaatatg cgctccttct tctggcgggc ccagagcatc ctctccagtc
      481 cttgactcac cagatctgct ctgaatctgc tgtccaaaac cttccaccaa acttggtgtt
      541 tcctcctctt gctctgctct gcctgagcct gctcaccact gctctcattc tcagctgtgt
      601 ggtcggagtg cttctcactg actgcgtcca gcagggccag tagctgaggc tgtgaagccg
      661 ctggaagcac cacactcagg agtcgcagta gtgtgctgcc tggtaccatt gtcatccttg
      721 agaggtatga caccagcctc agtccctggc tgcaaaggct ggccatggtg tcagcttcaa
      781 tgctttccag agaatcttcc agcaccgcac cgttctgcca ctcctctctc accctctgct
      841 gatgctgctc cagc
//
