LOCUS       sequence-55653-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2ala7-Flag
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(134..157)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag
       61 actcgagcgg ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggtctag
      121 atatctcgac tcacttgtca tcgtcgtcct tgtaatctcc gccagatcct ggtgttccgc
      181 cacgcgtgtc caagcccaag gccctgttgg ccttcttggc attcaggaaa ttctttttcc
      241 tcctgggagg aacacgcaaa gagatctcgg gtgcagctgc agctctgaat acaaagatct
      301 tttctcccgt gtttaatatg tccatagcgt ctgccccagt gatggggatg ggagccagtt
      361 ctccaatggg ttctagggac aggtgtggcc agcttccttt cccaggaaac atcaccctct
      421 cctggacggg aacatatatc ttcttcaata tgcgctcctt cttctggcgg gcccagagca
      481 tcctctccag tccttgactc accagatctg ctctgaatct gctgtccaaa accttccacc
      541 aaacttggtg tttcctcctc ttgctctgct ctgcctgagc ctgctcacca ctgctctcat
      601 tctcagctgt gtggtcggag tgcttctcac tgactgcgtc cagcagggcc agtagctgag
      661 gctgtgaagc cgctggaagc accacactca ggagtcgcag tagtgtgctg cctggtacca
      721 ttgtcatcct tgagaggtat gacaccagcc tcagtccctg gctgcaaagg ctggccatgg
      781 tgtcagct
//
