LOCUS       sequence-55680-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, 
            Svoboda K.
  TITLE     A genetically encoded fluorescent sensor of ERK activity
  JOURNAL   PNAS 2008 Dec 9;105(49):19264-9
  PUBMED    19033456
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PNAS"; 
            date: "2008-12-9"; volume: "105(49)"; pages: "19264-"
COMMENT     Sequence Label: cytoplasmic EKAR (EGFP-mRFP)
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          133..265
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     248..267
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        310..328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(464..482)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct
       61 aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      121 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      181 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      241 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      301 tagagtactt aatacgactc actataggct agcatggcct cctccgagga cgtcatcaag
      361 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      421 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      481 aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggctcc
      541 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      601 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      661 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg ca
//
