LOCUS       sequence-55689-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pCS2 YFP-Smo-Weyer's
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     39..56
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(263..265,266..268,269..781)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(308..329)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(569..588)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata
       61 caagctactt gttctttttg caggatccca tcgattcgaa ttcaccatgg ccgctggccg
      121 ccccgtgcgt gggcccgagc tggcgccccg gaggctgctg cagttgctgc tgctggtact
      181 gctggggggc ccgggccggg gggcggcctt gagcgggaac gtgaccgggc ctgggcctca
      241 cagcgccagc gggagctcga gcatggtgag caagggcgag gagctgttca ccggggtggt
      301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      421 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
      481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccc
//
