LOCUS       sequence-55693-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     Renhao Lab chicken GPIb-IX plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1(-)-chicken Flag-GPIba
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc gccaccatgg ccgtgcggtt tcccgctctg
      121 ctcccgctga tcctcgcagc cctgcagccc gcagccgtcc ccgcagagcc ggattacaag
      181 gatgacgatg acaagggagg aggagagccg ccgccgccct gcccgtggga gatgaacaag
      241 gtgaaggaca tcgtggaggt gaactgcacc gggcgggatc tcggcgcggt gcccgccggc
      301 ctgcccgagg acacgggcat cctgctgctc aacgccaacc gcctgctgtt cgtctccacg
      361 gccgccttcc agaagctggc cgccctgcag gacctcgacc tgtctgacaa cggcctgcag
      421 gctgtgctca ctgaggccgc gctgcccagc ctgaaggagc tgctgctgtc ccacaacgcg
      481 ctggaggctc tgcccgccct gcagggcctg cccgcgctca cccgcctggc cctggctcac
      541 aacaagctgc aggctctggc tcccgcggcc ttccgtgtcg tttcgcagct gcaggacctg
      601 gacctgcggg gcaacgcgct gcggacactc cccgaggacg tcttcatggg gctgcgagcg
      661 ctgaaggact tggatctgtc ggacaacgcc ttggaggagc tgcccagagg gctgctgcag
      721 gacctggtga cgctggagac gctgtggctg tcggggaacc ggctccgcgc cctgccctcc
      781 ggcttcttcc ccgagg
//
