LOCUS       sequence-55711-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-BBL1-ID14
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        140..244
                     /gene="bla"
                     /label=AmpR promoter
     promoter        246..603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        246..437
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(269..289)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      454..589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(692..711)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc gaatgctgac
       61 acccgaccaa ggtacctccg gatcctggct gcaggttgtc gccagcattc cgtcaggtgg
      121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      241 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      301 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      361 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      421 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      481 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      541 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      601 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      661 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      721 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      781 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
      841 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagc
//
