LOCUS       sequence-55714-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-BBL1-ID32
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        153..257
                     /gene="bla"
                     /label=AmpR promoter
     promoter        259..616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        259..450
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(282..302)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      467..602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(705..724)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc gaatgctgcc
       61 aggcacacgg gctgcagtcc ggatcctggg gtaccggttg ctccctgtct tgtgtcagca
      121 ttccgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      181 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      241 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
      301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      361 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      421 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
      481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      601 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
      661 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      721 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      781 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
      841 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      901 tcactga
//
