LOCUS       sequence-55727-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TALEN-ID12-T
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        106..124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             173..238
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             245..265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttttgga ccctcgtaca gaagctaata cgactcacta
      121 tagggaaata agagagaaaa gaagagtaag aagaaataat agactcgaga tggactacaa
      181 ggaccacgac ggcgactaca aggaccacga catcgactac aaggacgacg acgacaagat
      241 ggcccccaag aagaagagaa aggtgggcat ccacagaggc gtgcccatgg tggacctgag
      301 aaccctgggc tacagccagc agcagcagga gaagatcaag cccaaggtga gaagcaccgt
      361 ggcccagcac cacgaggccc tggtgggcca cggcttcacc cacgcccaca tcgtggccct
      421 gagccagcac cccgccgccc tgggcaccgt ggccgtgaag taccaggaca tgatcgccgc
      481 cctgcccgag gccacccacg aggccatcgt gggcgtgggc aagcagtgga gcggcgccag
      541 agccctggag gccctgctga ccgtggccgg cgagctgaga ggcccccccc tccagctgga
      601 caccggccag ctgctgaaga tcgccaagag aggcggcgtg accgccgtgg aggccgtgca
      661 cgcctggaga aacgccctga ccggcgcccc cctgaatttg acacccgacc aaggtaccgg
      721 tggatccggt ggtaccggtt gctccctgtc ttgtgtcaag accatgggct gaccc
//
