LOCUS       sequence-55731-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_307
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(101..107,108..125,126..131))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(156..280)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          309..512
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(332..352)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
       61 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgatcaatcg atgctagcct
      301 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
      361 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
      421 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
      481 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
      541 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
      601 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca
      661 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
      721 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca
      781 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
      841 catgtgcgct gggacgcacg ggcc
//
