LOCUS       sequence-55732-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_305
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(101..107,108..125,126..131))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(156..280)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(301..801)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(364..382)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
       61 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca gcttttttgt acaaacttgt tgatcaatcg atgctagccc
      301 ctggggagag aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg
      361 aggcttgcag aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca
      421 ccccgcctcc cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc
      481 gctattggcc acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc
      541 tgcgagggta ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg
      601 caaggaacct tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt
      661 agcggcgaag atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc
      721 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
      781 gctgccttgg aaaaggcgca acctg
//
