LOCUS       sequence-55774-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YFP-PIF for red-light-induced binding to Phy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL175
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..236)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      32..167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     342..361
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        436..540
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(760..779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctcgccctt gctcaccatg gtaccagcct aggcctccaa aaaagcctcc tcactacttc
       61 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
      121 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
      181 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgcctca
      241 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
      301 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
      361 actgagagtg caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact
      421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      721 gaagaacgtt ttccaatgat gagcactttt taaagttctg ctatgtggcg cggtattatc
      781 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      841 gg
//
