LOCUS       sequence-55775-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for making transgenic mice. Contains a loxP-flanked cassette 
            with a PGK-neo selectable marker and a tpA transcriptional stop 
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, 
            Costantini F
  TITLE     Cre reporter strains produced by targeted insertion of EYFP and ECFP
            into the ROSA26 locus.
  JOURNAL   BMC Dev Biol. 2001;1:4. doi: 10.1186/1471-213x-1-4. Epub 2001 Mar 
            27.
  PUBMED    11299042
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2001"; volume: "1:4. doi"; pages: " 
            10.1186/1471-213x-1-4. Epub 2001 Mar 27"
COMMENT     Sequence Label: pBigT
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    31..255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     244..261
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     298..314
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     299..315
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(350..383)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    412..546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    666..800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
       61 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
      121 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
      181 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
      241 aactagaagg cacagtcgag gctgatcagc gagctcgcgg ccgcccgcgg gggcccctcg
      301 aggtcgacgg tatcgataag ctagcttggg ctgcaggtcg agggacctaa taacttcgta
      361 tagcatacat tatacgaagt tatattaagg gttccggatc agcttgatgg ggatccagac
      421 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      481 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      541 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      601 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagag
      661 tcgcagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      721 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      781 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttc
//
