LOCUS       sequence-55787-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Wright DJ, King K, Modrich P
  TITLE     The negative charge of Glu-111 is required to activate the cleavage 
            center of EcoRI endonuclease.
  JOURNAL   J Biol Chem. 1989 Jul 15;264(20):11816-21.
  PUBMED    2745418
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1989-07-15"; volume: "264(20)"; pages: "11816-21"
COMMENT     Sequence Label: pEQ111m
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(469..488)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtactgaggc gagaattgat tctaatggta atagaattat ctcgccaaac aactgcttat
       61 ggctaactaa cctagatgtc tttattaggc ataaagactt gcctcttaca agaaaatatt
      121 ttgggaatga aagttcatat ccaaaatatg ataattatga tgctataaat gtaaacaaaa
      181 caaaggatat tccattagat tacaatgggg ttatgggggt tcctatcaca ttcttgcata
      241 agtttaaccc tgagcaattt gagttaataa aatttagaaa gggtgttgat gaaaaagatt
      301 tgtctataaa tggtaaatgc ccttatttca gaattttgat aaaaaacaaa cgattacaaa
      361 agtaattgat gtttgttagt tttttcttga gatcattagc ttcccggatc ctctacgccg
      421 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      481 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      541 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      601 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      661 aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
      721 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
      781 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
      841 gctggagcgc gacgatgatc ggcctgtcgc ttggcggtat tcggaatctt gcacgccctc
      901 gctcaagcct tcgtcactgg tccgcaccaa cga
//
