LOCUS       sequence-55837-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-GYG
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..69,70..75,76..92,93..99)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             166..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa
       61 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      121 caatttcaca cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat
      181 caccatagcg attacgacat ccccactact gagaatcttt attttcaggg atccacagat
      241 caggcctttg tgacactaac cacaaacgat gcctacgcca aaggtgccct ggtcctggga
      301 tcatctctga aacagcacag gaccaccagg aggctggtcg tgctcgccac ccctcaggtc
      361 tcagactcca tgagaaaagt tttagagaca gtctttgatg aagtcatcat ggtagatgtc
      421 ttggacagtg gcgattctgc tcatctaacc ttaatgaaga ggccagagtt gggtgtcacg
      481 ctgacaaagc tccactgctg gtcgcttaca cagtattcaa aatgtgtatt catggatgca
      541 gatactctgg tcctagcaaa tattgatgat ctttttgaca gagaagaatt gtcagcagca
      601 ccagacccag ggtggcctga ctgcttcaat tccggagtct tcgtttatca gccttcagtt
      661 gaaacataca atcagctgtt gcatcttgct tctgagcaag gtagttttga tggtggggac
      721 caaggcatac tgaacacatt ttttagcagc tgggcaacaa cagatatcag aaaacacctg
      781 ccgtttattt ataacctaag cagcatctct atatactcct acctcccggc atttaaagtg
      841 tttggtgcaa gtgccaaagt tgtgcatttc ctgggacgag tcaaaccatg gaa
//
