LOCUS       sequence-55838-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-PPP1R1A
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..69,70..75,76..92,93..99)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             166..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      790..883
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa
       61 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      121 caatttcaca cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat
      181 caccatagcg attacgacat ccccactact gagaatcttt attttcaggg atctgagcaa
      241 gacaacagcc cccgaaagat ccagttcacg gtcccgctgc tggagccgca ccttgacccc
      301 gaggcggcgg agcagattcg gaggcgccgc cccacccctg ccaccctcgt gctgaccagt
      361 gaccagtcat ccccagagat agatgaagac cggatcccca acccacatct caagtccact
      421 ttggcaatgt ctccacggca acggaagaag atgacaagga tcacacccac aatgaaagag
      481 ctccagatga tggttgaaca tcacctgggg caacagcagc aaggagagga acctgagggg
      541 gccgctgaga gcacaggaac ccaggagtcc cgcccacctg ggatcccaga cacagaagtg
      601 gagtcaaggc tgggcacctc tgggacagca aaaaaaactg cagaatgcat ccctaaaact
      661 cacgagagag gcagtaagga acccagcaca aaagaaccct caacccatat accaccactg
      721 gattccaagg gagccaactc ggtctgagcg gccgctcgac ctgcagccaa gcttaattag
      781 ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
      841 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaa
//
