LOCUS       sequence-55840-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-SCP2
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             75..86
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             99..119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atttgctttg tgagcggata acaattataa tagattcaat tgtgagcgga taacaatttc
       61 acacagaatt cattaaagag gagaaattaa ctatgaaaca tcaccatcac catcaccata
      121 gcgattacga catccccact actgagaatc tttattttca gggatccggt tttccggaag
      181 ccgccagttc ttttagaact catcaaattg aagctgttcc aaccagctct gcaagtgatg
      241 gatttaaggc aaatcttgtt tttaaggaga ttgagaagaa acttgaagag gaaggggaac
      301 agtttgtgaa gaaaatcggt ggtatttttg ccttcaaggt gaaagatggc cctgggggta
      361 aagaggccac ctgggtggtg gatgtgaaga atggcaaagg atcagtgctt cctaactcag
      421 ataagaaggc tgactgcaca atcacaatgg ctgactcaga cttcctggct ttaatgactg
      481 gtaaaatgaa tcctcagtcg gccttctttc aaggcaaatt gaaaatcact ggcaacatgg
      541 gtctcgctat gaagttacaa aatcttcagc ttcagccagg caacgctaag ctctgaagaa
      601 ctccctttgg ctacttttga aaatcaagat gagatatata gatatatatc catacatttt
      661 attgtcagaa tttagactga aactacacat tggcaaatag cgtgggatag atttgtttct
      721 taatgggtgt ga
//
