LOCUS       sequence-55841-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Bussow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork 
            P, Lehrach H, Heinemann U
  TITLE     Structural genomics of human proteins--target selection and 
            generation of a public catalogue of expression clones.
  JOURNAL   Microb Cell Fact. 2005 Jul 5;4:21. doi: 10.1186/1475-2859-4-21.
  PUBMED    15998469
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2005-07-5"; volume: "4:21. doi"; pages: " 
            10.1186/1475-2859-4-21"
COMMENT     Sequence Label: pQTEV-TCTEL1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..69,70..75,76..92,93..99)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             166..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaatcataa
       61 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      121 caatttcaca cagaattcat taaagaggag aaattaacta tgaaacatca ccatcaccat
      181 caccatagcg attacgacat ccccactact gagaatcttt attttcaggg atccgaagac
      241 taccaggctg cggaggagac tgcttttgtt gttgatgaag tgagcaacat tgtaaaagag
      301 gctatagaaa gcgcaattgg tggtaacgct tatcaacaca gcaaggtgaa ccagtggacc
      361 acaaatgtag tagaacaaac tttaagccaa ctcaccaagc tgggaaaacc atttaaatac
      421 atcgtgacct gtgtaattat gcagaagaat ggagctggat tacacacagc aagttcctgc
      481 ttctgggaca gctctactga cgggagctgc actgtgcgat gggagaataa gaccatgtac
      541 tgcatcgtca gtgccttcgg actgtctatt tgacctgcag tccagcctat ggcctttctc
      601 cttttgtctc tagttcatcc tctaaccacc agccatgaat tcagtgaact cttttctcat
      661 tctctttgtt ttgtggcact ttcacaatgt agaggaaaaa accaaatgac cgcactgtga
      721 tgtgaatggc accgaagtca gatgagtatc cctgtaggtc acctgcagcc tgcgttgcca
      781 cttgtcttaa ctctgaatat ttcatttcaa aggtgctaaa atctgaaatc tgctagtgtg
      841 aaacttgctc tactctctga aatgattcaa atacactaat tttccatact ttatactt
//
