LOCUS       sequence-55856-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD
  TITLE     Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and
            Mammals.
  JOURNAL   Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 
            10.1016/j.cell.2012.09.027.
  PUBMED    23063653
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2012 
            Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027."
COMMENT     Sequence Label: pcold-6xHis-HRV3Csite-DmLoqsPA
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..47
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             84..107
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ccatgaatca caaagtgcat catcatcatc atcatatcga aggtaggcat atggagctcg
       61 gtaccctcga gggatccgaa ttccttgaag tcctctttca gggacccgtc gacatggacc
      121 aggagaattt ccacggctcc agcttgccgc agcagctaca gaacctccac atccagccgc
      181 agcaggcgtc ccccaatcct gtccagacgg gatttgctcc acggcggcac tataataacc
      241 ttgtcggcct gggcaatgga aatgccgtca gtggtagtcc ggtgaagggt gctccgctgg
      301 ggcagcgcca tgtgaagctc aagaaggaga agatatccgc ccaggttgcg cagctgtctc
      361 agccaggtca gctgcagctg tcagatgttg gtgatcctgc cttggcgggc ggatcgggct
      421 tacaaggtgg agtcggcctt atgggcgtaa tattgcccag cgacgaggcc ttaaagttcg
      481 tcagcgaaac ggacgccaat ggactggcca tgaagacgcc cgtcagcatt ctgcaagagc
      541 tgctaagccg acgaggaatt actcccggct atgaacttgt ccagatcgag ggcgccatac
      601 atgagccgac cttccggttt cgcgtttcct ttaaggacaa ggatacgccc ttcacggcca
      661 tgggggcagg acgatcgaag aaggaggcca agcatgcggc ggcccgtgcg ctcatcgaca
      721 agctgatcgg cgcgcagctg ccggaatcgc ctagcagctc cgctggtccg tcggtgactg
      781 ggctcacggt cgccggaagc ggaggagacg gcaatgccaa tgccacaggc ggaggagatg
      841 ccagcgacaa gaccgttggt aatccgattg gctggttgca ggaaatgtgc atgcaacggc
      901 gatggccacc gccgtcgtac gaaacggaaa cggaagtggg tcttccc
//
