LOCUS       sequence-55860-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD
  TITLE     Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and
            Mammals.
  JOURNAL   Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 
            10.1016/j.cell.2012.09.027.
  PUBMED    23063653
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2012 
            Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027."
COMMENT     Sequence Label: pcold-6xHis-HRV3Csite-HsPACT
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..17
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..47
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             84..107
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ccatgaatca caaagtgcat catcatcatc atcatatcga aggtaggcat atggagctcg
       61 gtaccctcga gggatccgaa ttccttgaag tcctctttca gggacccgtc gacatgtccc
      121 agagcaggca ccgcgccgag gccccgccgc tggagcgcga ggacagtggg accttcagtt
      181 tggggaagat gataacagct aagccaggga aaacaccgat tcaggtatta cacgaatacg
      241 gcatgaagac caagaacatc ccagtttatg aatgtgaaag atctgatgtg caaatacacg
      301 tgcccacttt caccttcaga gtaaccgttg gtgacataac ctgcacaggt gaaggtacaa
      361 gtaagaagct ggcgaaacat agagctgcag aggctgccat aaacattttg aaagccaatg
      421 caagtatttg ctttgcagtt cctgacccct taatgcctga cccttccaag caaccaaaga
      481 accagcttaa tcctattggt tcattacagg aattggctat tcatcatggc tggagacttc
      541 ctgaatatac cctttcccag gagggaggac ctgctcataa gagagaatat actacaattt
      601 gcaggctaga gtcatttatg gaaactggaa agggggcatc aaaaaagcaa gccaaaagga
      661 atgctgctga gaaatttctt gccaaattta gtaatatttc tccagagaac cacatttctt
      721 taacaaatgt agtaggacat tctttaggat gtacttggca ttccttgagg aattctcctg
      781 gtgaaaagat caacttactg aaaagaagcc tccttagtat tccaaataca gattacatcc
      841 agctgcttag tgaaattgcc aaggaacaag
//
