LOCUS       sequence-55865-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-1CA2
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     52..68
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     53..69
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..393)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(373..391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(412..428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(412..428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(425..447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    436..452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(460..466,467..484,485..490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    505..526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(643..660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
       61 acggtatcga taagcttgat atttgggaat gcagacacaa gacagggagc aaccgttgca
      121 ctgtttccag ggcctgctta ccgccgatgt tggaggcgat ggcgaccact tgtgcagggg
      181 tcaaaccgtg ggcttgacac aacacgggaa ggagccgttg gacagtctcc aatgcttgtt
      241 tccctccgtc atggctggca atggccacaa cttggtcggg tgtctgcatt catttggatc
      301 gaattcctgc agcccggggg atccgcccgg gctagagcgg ccgccaccgc ggtggagctc
      361 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      841 gcatcacaaa aatcgacgct caagtc
//
