LOCUS       sequence-55874-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-1TG2
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(307..323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(307..323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(320..342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    331..347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(355..361,362..379,380..385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    400..421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(538..555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(789..808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcagacac ccgaccaagt
       61 tgtggccatt gccagcaacg gtggagggaa acaagcattg gagactgtcc aacggctcct
      121 tcccgtgttg tgtcaagccc acggtttgac ccctgcacaa gtggtcgcca tcgcctccaa
      181 caagggcggt aagcaggccc tggaaacagt gcaacggttg ctccctgtct tgtgtctgca
      241 ttcccaaata tcggatcccg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta
      301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      901 tcatagctca cgctgtaggt atctcagttc ggtgtag
//
