LOCUS       sequence-55876-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-3AA4
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     52..68
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     53..69
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..444)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(424..442)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(463..479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(463..479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(476..498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    487..503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(511..517,518..535,536..541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    556..577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(694..711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
       61 acggtatcga taagcttgat atttgggaat gcaggcgaca acctggtctg gggtcagtcc
      121 atgatcctgg cacagcacag gcagcaggcg ctgcactgtt tccagggcct gcttaccgcc
      181 gatgttggag gcgatggcga ccacttgtgc aggggtcaaa ccgtgggctt gacacaacac
      241 gggaaggagc cgttggacag tctccaatgc ttgtttccct ccgatgttgc tggcaatggc
      301 cacaacttga tcgggagtaa gcccgtgtgc ctggctgcat tcatttggat cgaattcctg
      361 cagcccgggg gatccgcccg ggctagagcg gccgccaccg cggtggagct ccagcttttg
      421 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      481 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      541 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      601 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      661 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
//
