LOCUS       sequence-55891-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-3TT4
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(358..374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(358..374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(371..393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    382..398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(406..412,413..430,431..436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    451..472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(589..606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(840..859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atatttggga atgcaggcga caacctggtc
       61 tggggtcagt ccatgatcct ggcacagcac aggcagcagg cgctgcactg tttccagggc
      121 ctgcttaccg ccaccgttgg aggcgatggc gaccacttgt gcaggggtca aaccgtgggc
      181 ttgacacaac acgggaagga gccgttggac agtctccaat gcttgtttcc ctccaccgtt
      241 gctggcaatg gccacaactt gatcgggagt aagcccgtgt gcctggctgc attcatttgg
      301 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc
      361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      901 gcttaccgga tacctgtccg cctttctccc ttcgg
//
