LOCUS       sequence-55904-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-2CC3
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     52..68
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     53..69
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..440)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(420..438)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(459..475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(459..475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(472..494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    483..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(507..513,514..531,532..537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    552..573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(690..707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
       61 acggtatcga taagcttgat atttgggaat gcagcccgtg tgcctggcag agaactggga
      121 gaagtctctg aacagtctca agagcctgtt tgccaccgtc atgtgatgca atggccacga
      181 cttgctcggg tgtaaggccg tggtcttgac aaaggacggg caacaacctt tgcacggttt
      241 ccagggcttg ctttcccccg tcatgagagg cgattgcgac cacctggtct ggggtcagtc
      301 catgatcttg acacaagaca gggagcaacc gggcattcat ttggatcgaa ttcctgcagc
      361 ccgggggatc cgcccgggct agagcggccg ccaccgcggt ggagctccag cttttgttcc
      421 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
      481 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      541 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      601 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      901 cgacgctcaa gtcagaggtg gcgaaacc
//
