LOCUS       sequence-55914-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-2TT3
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(354..370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(354..370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(367..389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    378..394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(402..408,409..426,427..432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    447..468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(585..602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(836..855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcccggtt gctccctgtc
       61 ttgtgtcaag atcatggact gaccccagac caggtggtcg caatcgcctc taacggtggg
      121 ggaaagcaag ccctggaaac cgtgcaaagg ttgttgcccg tcctttgtca agaccacggc
      181 cttacacccg agcaagtcgt ggccattgca tcaaacggtg gtggcaaaca ggctcttgag
      241 actgttcaga gacttctccc agttctctgc caggcacacg ggctgcattc ccaaatatcg
      301 gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag
      361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tag
//
