LOCUS       sequence-55916-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-4AC1
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(321..337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(321..337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(334..356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    345..361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(369..375,376..393,394..399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    414..435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(552..569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(803..822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcaggttg tcgccatcgc
       61 ctctaacatc gggggaaagc aagccctgga aaccgtgcaa aggttgttgc ccgtcctttg
      121 tcaagaccac ggccttacac ccgagcaagt cgtggccatt gcatcacatg acggtggcaa
      181 acaggctctt gagactgttc agagacttct cccagttctc tgtcaagccc acggtttgac
      241 acccgaccaa gggcattccc aatatcggat cccgggcccg tcgactgcag aggcctgcat
      301 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca
//
