LOCUS       sequence-55923-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-4GA1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(322..338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(335..357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    346..362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(370..376,377..394,395..400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    415..436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(553..570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(804..823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagctcgg tacctcgcga atgcatctag atccaaatga atgcaggttg tcgccatcgc
       61 ctctaacaag gggggaaagc aagccctgga aaccgtgcaa aggttgttgc ccgtcctttg
      121 tcaagaccac ggccttacac ccgagcaagt cgtggccatt gcatcaaaca tcggtggcaa
      181 acaggctctt gagactgttc agagacttct cccagttctc tgtcaagccc acggtttgac
      241 acccgaccaa gggcattccc aaatatcgga tcccgggccc gtcgactgca gaggcctgca
      301 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt a
//
