LOCUS       sequence-55942-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Blakeley BD, Chapman AM, McNaughton BR
  TITLE     Split-superpositive GFP reassembly is a fast, efficient, and robust 
            method for detecting protein-protein interactions in vivo.
  JOURNAL   Mol Biosyst. 2012 Aug;8(8):2036-40. Epub 2012 Jun 12.
  PUBMED    22692102
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biosyst."; date: "2012-08"; volume: "8(8)"; pages: "2036-40. Epub 
            2012 Jun 12"
COMMENT     Sequence Label: pET11a-Z-NspGFP
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..71)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(624..641)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             655..677
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    692..716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(716..735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(717..735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(819..838)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ccttactgcg ccagttcctt tttcagagct
       61 tgtaactccc acttcagctg cgcaagttct tttttgtttg cctgcaattc ttttttgagg
      121 gcgctcgagc cagagccaga gccaccgcgt ttatcggccg taatatacac cttatgagaa
      181 ttaaagttat aacgcagttt atggcctaaa atgttgcctt tttctttgaa gtcacgacct
      241 ttcagtttga tacgatttac tagtgtgcgg ccttcaaatt tcacttccgc acgcgtttta
      301 tatttgccat ccttcttgaa gctgattgta cgctcttgaa catatccttt aggcatggct
      361 gatttgaaaa aatcgtgacg tttcatatgt ttagggtaac gactgaagca ctgaacgcca
      421 taggtcaggg tggtcactaa ggtgggccac ggaacgggta atttgcctgt tgtgcaaata
      481 aacttcaggg tcaatttacc acgggtagcg tcacctttgc cttcgccgcg cacgctaaat
      541 ttatgaccgt tcacgtcgcc ctttaattcc actaagatcg gtactttacc acgaaacaga
      601 cgttcacctt tgctagcgcc accgtgatgg tggtggtgat gacccatatg tatatctcct
      661 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta
      721 tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt
      781 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      841 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      901 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      961 ggcggcggtg ctcaacggcc tcaacctact a
//
