LOCUS       sequence-55960-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: aTRE
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    175..266
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(207..226)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    280..328
                     /note="synthetic polyadenylation signal"
     promoter        401..505
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(724..743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgctcacca tggtggcgac cggtgcgcgc ataaagcgcc tcgcaaatcg cgtccgacgg
       61 gcaactaacg gctccggtca agcttccatt atatagaatt ctgaggtcaa gttacggatc
      121 cgtaacttga cctcagctga cctcaataac tcgagcaatt ggtcgactat cgatagagaa
      181 atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg
      241 ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca
      301 gatgtaatga aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat
      361 attacttctc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      481 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      781 catacactat tctcagaatg acttgg
//
