LOCUS       sequence-55962-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: rME
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    154..245
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(186..205)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    259..307
                     /note="synthetic polyadenylation signal"
     promoter        380..484
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(703..722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgctcaccat ggtggcgacc ggtgcgcgca taaagcgcct cgcaaatcgc gtccgacggg
       61 caactaacgg ctccggtcaa gcttccatta tatagaattc cactgtcctc ccctaacccc
      121 aacgtcctct cgagcaattg gtcgactatc gatagagaaa tgttctggca cctgcacttg
      181 cactggggac agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt
      241 atgttagtac tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata
      301 ttttattgcg gccgctccaa gtacctcccg taccttaata ttacttctcg acccaggtgg
      361 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      421 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      481 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      541 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      601 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      661 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      721 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      781 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      841 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac t
//
