LOCUS       sequence-55969-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: 1XDR4
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    168..259
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(200..219)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    273..321
                     /note="synthetic polyadenylation signal"
     promoter        394..498
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(717..736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtgcgcgca taaagcgcct cgcaaatcgc
       61 gtccgacggg caactaacgg ctccggtcaa gcttccatta tatagaattc gaagctggtg
      121 acctgaagtg acctgaagct ggctcgagca attggtcgac tatcgataga gaaatgttct
      181 ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt tcgttttgtt
      241 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
      301 tgaaaataaa gatattttat tgcggccgct ccaagtacct cccgtacctt aatattactt
      361 ctcgacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
      781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tac
//
