LOCUS       sequence-55971-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: rGH-PAL
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    151..242
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(183..202)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    256..304
                     /note="synthetic polyadenylation signal"
     promoter        377..481
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(700..719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgctcacca tggtggcgac cggtgcgcgc ataaagcgcc tcgcaaatcg cgtccgacgg
       61 gcaactaacg gctccggtca agcttccatt atatagaatt cgaagctggt gaggtcatga
      121 cctcactcga gcaattggtc gactatcgat agagaaatgt tctggcacct gcacttgcac
      181 tggggacagc ctattttgct agtttgtttt gtttcgtttt gttttgatgg agagcgtatg
      241 ttagtactat cgattcacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt
      301 tattgcggcc gctccaagta cctcccgtac cttaatatta cttctcgacc caggtggcac
      361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      901 cggaggaccg a
//
