LOCUS       sequence-55972-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: cLS-F2
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    165..256
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(197..216)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    270..318
                     /note="synthetic polyadenylation signal"
     promoter        391..495
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(714..733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtgcgcgca taaagcgcct cgcaaatcgc
       61 gtccgacggg caactaacgg ctccggtcaa gcttccatta tatagaattc cgtaacttga
      121 cctcagctgg ggtcaataac tcgagcaatt ggtcgactat cgatagagaa atgttctggc
      181 acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg
      241 atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga
      301 aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat attacttctc
      361 gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      841 acagtaagag aattatgcag tgctgccata accatgagtg a
//
