LOCUS       sequence-55973-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Billings NA, Emerson MM, Cepko CL
  TITLE     Analysis of thyroid response element activity during retinal 
            development.
  JOURNAL   PLoS One. 2010  . 5(10):e13739.
  PUBMED    21060789
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13739."
COMMENT     Sequence Label: rGH-ABC
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(199..218)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    272..320
                     /note="synthetic polyadenylation signal"
     promoter        393..497
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(716..735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtgcgc gcataaagcg cctcgcaaat cgcgtccgac
       61 gggcaactaa cggctccggt caagcttcca ttatatagaa ttcgaagctg gtgctgaggt
      121 cacgtccctg atcttacctt tctcgagcaa ttggtcgact atcgatagag aaatgttctg
      181 gcacctgcac ttgcactggg gacagcctat tttgctagtt tgttttgttt cgttttgttt
      241 tgatggagag cgtatgttag tactatcgat tcacacaaaa aaccaacaca cagatgtaat
      301 gaaaataaag atattttatt gcggccgctc caagtacctc ccgtacctta atattacttc
      361 tcgacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      721 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      781 attctcagaa tgacttg
//
