LOCUS       sequence-55985-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     John M Nickerson lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGIG-6xHIS.Mm.RPE65-eGFP
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattcaccac catgcatcac catcaccatc actctatcca aattgaacac
      121 cctgctggtg gctacaagaa actatttgaa actgtggagg aactgtcctc accactaaca
      181 gctcatgtca caggcaggat tcccctctgg ctcactggca gtctcctccg atgtgggcca
      241 gggctctttg aagttggatc tgagcctttc tatcacctgt ttgatggaca agcccttttg
      301 cacaagtttg acttcaagga gggccatgtc acataccaca gaagattcat ccgcactgat
      361 gcttatgttc gagcaatgac tgagaagagg attgtcataa cagaatttgg cacctgtgct
      421 ttcccagacc cctgcaagaa tatattttcc aggttttttt cttactttaa aggagtagag
      481 gttactgaca atgcccttgt aaatatctac ccagtgggag aagattacta tgcatgcaca
      541 gagaccaact ttatcacaaa gattaaccca gagaccttgg agacaattaa gcaggttgat
      601 ctttgcaact atatttctgt caatggtgcc actgctcatc cacatattga aagtgatgga
      661 acagtttaca acattgggaa ttgctttgga aaaaatttta cagttgccta caacattatt
      721 aa
//
