LOCUS       sequence-55991-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Miedel MT, Graf NJ, Stephen KE, Long OS, Pak SC, Perlmutter DH, 
            Silverman GA, Luke CJ
  TITLE     A pro-cathepsin L mutant is a luminal substrate for 
            endoplasmic-reticulum-associated degradation in C. elegans.
  JOURNAL   PLoS One. 2012;7(7):e40145. Epub 2012 Jul 2.
  PUBMED    22768338
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e40145. Epub 2012 Jul 2"
COMMENT     Sequence Label: pAV1997
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(243..259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(243..259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(256..278)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    267..283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(291..297,298..315,316..321))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    336..357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(474..491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(725..744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat gctagccaag
      121 ggtcctcctg aaaatgttct atgttatgtt agtatcattc gaaacatacc tttgggtcct
      181 ttggccaatc ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca
      241 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      301 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      361 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      421 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      781 ctgccgctta ccggat
//
